The bright way to detect cells secreting multiple cytokines.
The Fluorospot assay is in principle based on ELISpot but utilizes fluorescent-based detection systems, enabling the detection of cells secreting either of two different cytokines, or both, in the same well.
Fluorospot is in particular suitable for analysis of polyfunctional T cells, defined by their secretion of multiple cytokines, that have been suggested to be of high relevance for protection against infectious diseases e.g. HIV |
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